Pulsatilla grandis
Taxonomy: Angiospermae, Ranunculales, Ranunculaceae, Pulsatilla
Links: http://flora.nhm-wien.ac.at/Seiten-Arten/Pulsatilla-grandis.htm
Published: 2021-01-27
Pollen Description
Shape, Size and Aperture
pollen unit: monad, dispersal unit and peculiarities: monad, size (pollen unit): medium-sized (26-50 µm), size of hydrated pollen (LM): 36-40 µm, 41-50 µm, shortest polar axis in equatorial view (LM): 31-35 µm, longest polar axis in equatorial view (LM): 41-50 µm, shortest diameter in equatorial or polar view (LM): 36-40 µm, longest diameter in equatorial or polar view (LM): 36-40 µm, pollen class: colpate, polarity: isopolar, P/E-ratio: -, shape: spheroidal, outline in polar view: circular, dominant orientation (LM): oblique, P/E-ratio (dry pollen): prolate, shape (dry pollen): -, outline in polar view (dry pollen): lobate, infoldings (dry pollen): aperture(s) sunken, aperture number: 3, aperture type: colpus, aperture condition: colpate, tricolpate, aperture peculiarities: aperture membrane ornamented
Ornamentation and Structure
LM ornamentation LM: gemmate, verrucate, scabrate, nexine: -, sexine: -, SEM ornamentation SEM: microechinate, perforate, suprasculpture SEM: -, TEM tectum: eutectate, infratectum: columellate, foot layer: continuous, endexine: compact-continuous, intine: monolayered, wall peculiarities: -, supratectal element: -
Miscellaneous
pollen coatings: pollenkitt, reserves in cytoplasm: starch, lipids, cell number: 2-celled, Ubisch bodies: present
Author(s) of diagnosis: Oberschneider, Wolfgang; Auer, Waltraud
Pictures
1. flower(s)
2. flower(s)
3. upper focus
4. optical section
5. lower focus
6. generative cell (dark) and vegetative nucleus
7. hydrated pollen
8. hydrated pollen
9. hydrated pollen
10. polar view
11. polar view
12. equatorial view
13. detail of aperture
14. exine surface
15. polar view of dry pollen grain
16. dry pollen grain in equatorial view
17. interapertural area of pollen wall
18. aperture
19. pollen wall and tapetum cells with Ubisch bodies (asterisk)
Picture legend
- flower(s), photographer: Svojtka, M.
- flower(s), photographer: Svojtka, M.
- upper focus - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
- optical section - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
- lower focus - fresh, acetolyzed, unstained, photographer: Oberschneider, W.
- generative cell (dark) and vegetative nucleus - fixed, formalin-acetic-alcohol, aceto-carmine, photographer: Oberschneider, W.
- hydrated pollen - fresh, glycerine, unstained, photographer: Auer, W.
- hydrated pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
- hydrated pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
- polar view - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
- polar view - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
- equatorial view - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
- detail of aperture - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
- exine surface - fresh, rehydration (water) & chloroform & critical point dried & sputter coated with gold, photographer: Oberschneider, W.
- polar view of dry pollen grain - dry, sputter coated with gold, photographer: Oberschneider, W.
- dry pollen grain in equatorial view - dry, sputter coated with gold, photographer: Oberschneider, W.
- interapertural area of pollen wall - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Oberschneider, W.
- aperture - fresh, glutaraldehyde & osmium & potassium ferrocyanide, uranyl acetate & lead citrate, photographer: Oberschneider, W.
- pollen wall and tapetum cells with Ubisch bodies (asterisk) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Oberschneider, W.
Literature
- (1998) Preparing living pollen material for scanning electron microscopy using 2,2-dimethoxypropane (DMP) and criticalpoint drying. Biotechnic Histochem 73: 137–143
- (1994) Exkursionsflora von Österreich : 1180
Copyright and Citation
Cite this publication as:
Oberschneider W., Auer W., Svojtka M. 2021. Pulsatilla grandis. In: PalDat - A palynological database. https://pc8.botanik.univie.ac.at/pub/Pulsatilla_grandis/305136;jsessionid=9499000F4BDCCD05FC4CC00B0745DF98; accessed 2025-01-25