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PalDat - Palynological Database

an online publication on recent pollen

Helleborus viridis

Taxonomy: Angiospermae, Ranunculales, Ranunculaceae, Helleborus

Links: http://flora.nhm-wien.ac.at/Seiten-Arten/Helleborus-viridis.htm

Published: 2021-06-23

Pollen Description

Shape, Size and Aperture

pollen unit: monad,  dispersal unit and peculiarities: monad,  size (pollen unit): medium-sized (26-50 µm),  size of hydrated pollen (LM): 26-30 µm, 31-35 µm,  shortest polar axis in equatorial view (LM): 26-30 µm,  longest polar axis in equatorial view (LM): -,  shortest diameter in equatorial or polar view (LM): 31-35 µm,  longest diameter in equatorial or polar view (LM): 31-35 µm,  pollen class: colpate,  polarity: isopolar,  P/E-ratio: -,  shape: spheroidal,  outline in polar view: circular,  dominant orientation (LM): oblique,  P/E-ratio (dry pollen): prolate,  shape (dry pollen): -,  outline in polar view (dry pollen): circular,  infoldings (dry pollen): aperture(s) sunken,  aperture number: 3,  aperture type: colpus,  aperture condition: colpate, tricolpate,  aperture peculiarities: aperture membrane ornamented

Ornamentation and Structure

LM  ornamentation LM: reticulate,  nexine: -,  sexine: -,  SEM  ornamentation SEM: reticulate,  suprasculpture SEM: -,  TEM  tectum: semitectate,  infratectum: columellate,  foot layer: continuous,  endexine: compact-continuous,  intine: monolayered,  wall peculiarities: -,  supratectal element: -

Miscellaneous

pollen coatings: pollenkitt,  reserves in cytoplasm: starch, lipids,  cell number: 2-celled,  Ubisch bodies: present

Annotations: reticulum: size of lumina smaller around the colpium; thin endexine

Author(s) of diagnosis: Auer, Angelika; Auer, Waltraud

Pictures

flower(s)

1. flower(s)

polar view,upper focus

2. polar view, upper focus

optical section

3. optical section

polar view,lower focus

4. polar view, lower focus

pollen grain with generative cell (asterisk) and vegetative nucleus

5. pollen grain with generative cell (asterisk) and vegetative nucleus

hydrated Pollen

6. hydrated Pollen

hydrated Pollen

7. hydrated Pollen

hydrated Pollen

8. hydrated Pollen

polar view

9. polar view

equatorial view

10. equatorial view

detail of colpus membrane

11. detail of colpus membrane

exine surface

12. exine surface

polar view (dry pollen grain)

13. polar view (dry pollen grain)

dry pollen grain in equatorial view

14. dry pollen grain in equatorial view

interapertural area of pollen wall

15. interapertural area of pollen wall

aperture, intine (I), endexine (E)

16. aperture, intine (I), endexine (E)

tapetum cells with Ubisch bodies (U)

17. tapetum cells with Ubisch bodies (U)

Picture legend

  1. flower(s), photographer: Auer, A.
  2. polar view, upper focus - fresh, acetolyzed, unstained, photographer: Auer, A.
  3. optical section - fresh, acetolyzed, unstained, photographer: Auer, A.
  4. polar view, lower focus - fresh, acetolyzed, unstained, photographer: Auer, A.
  5. pollen grain with generative cell (asterisk) and vegetative nucleus - fixed, formalin-acetic-alcohol, aceto-carmine, photographer: Auer, A.
  6. hydrated Pollen - fresh, glycerine, unstained, photographer: Auer, W.
  7. hydrated Pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
  8. hydrated Pollen - fresh, glycerine, ruthenium red, photographer: Auer, W.
  9. polar view - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Auer, A.
  10. equatorial view - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Auer, A.
  11. detail of colpus membrane - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Auer, A.
  12. exine surface - fresh, rehydrated (water) & critical point dried & sputter coated with gold, photographer: Auer, A.
  13. polar view (dry pollen grain) - dry, sputter coated with gold, photographer: Auer, A.
  14. dry pollen grain in equatorial view - dry, sputter coated with gold, photographer: Auer, A.
  15. interapertural area of pollen wall - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Auer, A.
  16. aperture, intine (I), endexine (E) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, modified Thiéry-test, photographer: Auer, A.
  17. tapetum cells with Ubisch bodies (U) - fresh, glutaraldehyde & osmium & potassium ferrocyanide, uranyl acetate & lead citrate, photographer: Auer, A.

Literature

  • Echlin P, Godwin H (1968) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. I. The development of the tapetum and ubisch bodies. J Cell Sci 3: 161-174
  • Echlin P, Godwin H (1968) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. II. Pollen grain development through the callose special wall stage. J Cell Sci 3: 175-186
  • Echlin P, Godwin H (1969) The ultrastructure and ontogeny of pollen in Helleborus foetidus L. III. The formation of the pollen grain wall. J Cell Sci 5: 459-477
  • Heslop-Harrison J, Heslop-Harrison JS, Heslop-Harrison Y (1986) The comportment of the vegetative nucleus and generative cell in the pollen and pollen tubes of Helleborus foetidus L. Ann Bot 58: 1-12
  • Nowicke JW, Skvarla JJ (1983) A palynological study of the genus Helleborus (Ranunculaceae). Grana 22: 129-140
  • Oberschneider W (1998) Pollenmorphologie der Ranunculanae. Diplomarbeit. Universität Wien : 125 pp
  • Halbritter, H (1998) Preparing living pollen material for scanning electron microscopy using 2,2-dimethoxypropane (DMP) and criticalpoint drying. Biotechnic Histochem 73: 137–143

Copyright and Citation

Cite this publication as:

Auer A., Auer W. 2021. Helleborus viridis. In: PalDat - A palynological database. https://pc8.botanik.univie.ac.at/pub/Helleborus_viridis/306249;jsessionid=9CE67DFD31F1C61A69B39B5BE3C84184; accessed 2025-01-25

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